Coding

Part:BBa_K1480001:Design

Designed by: Roberto Alejandro Valderrama Alfaro   Group: iGEM14_UANL_Mty-Mexico   (2014-10-08)


Transcription Activator-Like Effector Nuclease (TALEN)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1270
    Illegal NheI site found at 1474
    Illegal NheI site found at 1882
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3019
    Illegal BamHI site found at 2425
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2959
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1138
    Illegal BsaI.rc site found at 790
    Illegal SapI.rc site found at 2439


Design Notes

Due the target sequence is based on a eukaryotic sequence, TALEN can bind to an undesirable site.


Source

Synthetic part based on an eukaryotic gene.

References